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HEMGN Polyclonal antibody

HEMGN Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 17616-1-AP

Print datasheet

Synonyms

EDAG, EDAG 1, HEMGN, hemogen, Hemopoietic gene protein, NDR, PRO1620

验证数据展示

  • WB analysis of Jurkat using 17616-1-AP

    WB analysis of Jurkat using 17616-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17616-1-AP

    IHC staining of human testis using 17616-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17616-1-AP (HEMGN antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 17616-1-AP

    IHC staining of human testis using 17616-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17616-1-AP (HEMGN antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HL-60 using 17616-1-AP

    IP experiment of HL-60 using 17616-1-AP

    IP result of anti-HEMGN (IP:17616-1-AP, 4ug; Detection:17616-1-AP 1:500) with HL-60 cells lysate 3000ug.

经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells, human testis tissue, mouse testis tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17616-1-AP targets HEMGN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen HEMGN fusion protein Ag11793 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hemogen
Synonyms EDAG, EDAG 1, HEMGN, hemogen, Hemopoietic gene protein, NDR, PRO1620
Calculated Molecular Weight 483 aa, 55 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC048324
Gene Symbol HEMGN
Gene ID (NCBI) 55363
RRIDAB_2117676
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXL5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HEMGN antibody 17616-1-APDownload protocol
IHC protocol for HEMGN antibody 17616-1-APDownload protocol
IP protocol for HEMGN antibody 17616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Biomedicines

Establishing Molecular Subgroups of CD8+ T Cell-Associated Genes in the Ovarian Cancer Tumour Microenvironment and Predicting the Immunotherapy Response

Authors - Yunshu Zhu
View Article