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验证数据展示

  • IHC staining of human breast cancer using 26635-1-AP

    IHC staining of human breast cancer using 26635-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26635-1-AP (HELZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 26635-1-AP

    WB analysis of HeLa using 26635-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26635-1-AP (HELZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26635-1-AP

    IF Staining of HepG2 using 26635-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HELZ antibody (26635-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

HELZ Polyclonal antibody

HELZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26635-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA0054, HUMORF5, DRHC, DHRC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26635-1-AP targets HELZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24326

Product name: Recombinant human HELZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-102 aa of BC130582

Sequence: MEDRRAEKSCEQACESLKRQDYEMALKHCTEALLSLGQYSMADFTGPCPLEIERIKIESLLYRIASFLQLKNYMQADEDCRHVLGEGLAKGEDAFRAVLCCM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 helicase with zinc finger
别名 KIAA0054, HUMORF5, DRHC, DHRC
计算分子量 1942 aa, 219 kDa
观测分子量 200 kDa
GenBank蛋白编号BC130582
基因名称 HELZ
Gene ID (NCBI) 9931
RRIDAB_2880584
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42694
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HELZ antibody 26635-1-APDownload protocol
IHC protocol for HELZ antibody 26635-1-APDownload protocol
IF protocol for HELZ antibody 26635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols