验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue, mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24730-1-AP targets HELT in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Ag20618 Product name: Recombinant human HELT protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 54-241 aa of BC140795 Sequence: ILEMTVQYLRALHSADFPRGREKELLAEFANYFHYGYHECMKNLVHYLTTVERMETKDTKYARILAFLQSKARLGAEPAFPPLGSLPEPDFSYQLHPAGPEFAGHSPGEAAVFPQGSGAGPFPWPPGAARSPALPYLPSAPVPLASPAQQHSPFLTPVQGLDRHYLNLIGHAHPNALNLHTPQHPPVL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | HES/HEY-like transcription factor |
| 别名 | HCM1228, HELT, HESL, Mgn |
| 计算分子量 | 327 aa, 36 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC140795 |
| 基因名称 | HELT |
| Gene ID (NCBI) | 391723 |
| RRID | AB_2879694 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A6NFD8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HELT is a transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'. HELT has two isoforms with MW 36 kDa and 27 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HELT antibody 24730-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
