验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MOLT-4 cells, EL-4 cells |
| Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87876-1-RR targets HELIOS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4087 Product name: Recombinant human IKZF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 227-526 aa of BC028936 Sequence: NNISLVPFERPAVIEKLTGNMGKRKSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALHPLMQHPPSTIAEVAPVISSAYSQVYHPNRIERPISRETADSHENNMDGPISLIRPKSRPQEREASPSNSCLDSTDSESSHDDHQSYQGHPALNPKRKQSPAYMKEDVKALDTTKAPKGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | IKAROS family zinc finger 2 (Helios) |
| 别名 | IKZF2, IKZF 2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2 |
| 计算分子量 | 500 aa, 57 kDa |
| 观测分子量 | 65-75 kDa |
| GenBank蛋白编号 | BC028936 |
| 基因名称 | HELIOS |
| Gene ID (NCBI) | 22807 |
| RRID | AB_3745802 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UKS7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HELIOS, encoded by the IKZF2 gene (Ikaros Family Zinc Finger Protein 2), is a transcription factor belonging to the Ikaros family of zinc-finger proteins. It is primarily expressed in hematopoietic cells, particularly in regulatory T cells (Tregs) and early lymphoid progenitors, where it plays a critical role in immune regulation, T-cell development, and the maintenance of Treg suppressive function. HELIOS binds to specific DNA sequences to regulate gene expression involved in lymphocyte differentiation and immune homeostasis. It is also widely used as a stable marker for activated Tregs. Dysregulation of HELIOS has been implicated in autoimmune diseases, allergy, and cancer immunotherapy resistance.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HELIOS antibody 87876-1-RR | Download protocol |
| WB protocol for HELIOS antibody 87876-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
