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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    WB analysis of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    IP experiment of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

HELIOS Monoclonal antibody, PBS Only

HELIOS Monoclonal Antibody for WB, IP, Indirect ELISA
Cat No. 68558-1-PBS

产品说明书

CloneNo. 1F1F10

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IP, Indirect ELISA

别名

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68558-1-PBS targets HELIOS in WB, IP, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag4357

Product name: Recombinant human IKZF2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 227-526 aa of BC028936

Sequence: NNISLVPFERPAVIEKLTGNMGKRKSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALHPLMQHPPSTIAEVAPVISSAYSQVYHPNRIERPISRETADSHENNMDGPISLIRPKSRPQEREASPSNSCLDSTDSESSHDDHQSYQGHPALNPKRKQSPAYMKEDVKALDTTKAPKGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 IKAROS family zinc finger 2 (Helios)
别名 HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2
计算分子量 500 aa, 57 kDa
观测分子量 65 kDa
GenBank蛋白编号BC028936
基因名称 HELIOS
Gene ID (NCBI) 22807
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UKS7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).