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验证数据展示

  • WB analysis of mouse brain using 25351-1-AP

    WB analysis of mouse brain using 25351-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25351-1-AP (HECTD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HECTD2 Polyclonal antibody

HECTD2 Polyclonal Antibody for WB, ELISA
Cat No. 25351-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

HECT domain containing 2, HECTD2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25351-1-AP targets HECTD2 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18360

Product name: Recombinant human HECTD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 428-776 aa of BC040187

Sequence: SDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITFDIRFPPCCYKKLLSPPIIPSGQNIPVGICNVTVDDLCQIMPELAHGLSELLSHEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKISVTNQNRKEYVQLYTDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPDLDMHALQRSTQYDGYAKTDLTIKYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNCLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HECT domain containing 2
别名 HECT domain containing 2, HECTD2
计算分子量 776 aa, 88 kDa
观测分子量 88 kDa
GenBank蛋白编号BC040187
基因名称 HECTD2
Gene ID (NCBI) 143279
RRIDAB_2880040
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5U5R9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HECTD2(HECT domain-containing protein 2) may be an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for HECTD2 antibody 25351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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