• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84918-5-RR

    WB analysis using 84918-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84918-5-RR (HECTD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84918-5-RR

    Affinity and Kinetic Characterization of 84918-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84918-5-RR against Human HECTD1 were performed. The affinity constant is 4.51 nM.

HECTD1 Recombinant antibody

HECTD1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84918-5-RR

产品说明书

CloneNo. 242344A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

HECT domain containing 1, EULIR, EC:2.3.2.26, E3 ubiquitin-protein ligase HECTD1, E3 ligase for inhibin receptor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, L02 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84918-5-RR targets HECTD1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 HECTD1 fusion protein Ag14581 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HECT domain containing 1
别名 HECT domain containing 1, EULIR, EC:2.3.2.26, E3 ubiquitin-protein ligase HECTD1, E3 ligase for inhibin receptor
计算分子量 2612 aa, 290 kDa
观测分子量 290 kDa
GenBank蛋白编号BC006237
基因名称 HECTD1
Gene ID (NCBI) 25831
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULT8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HECTD1 (E3 ubiquitin-protein ligase), which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates,may be required for development of the head mesenchyme and neural tube closure.It is also involved in sporadic Parkinson's disease(PMID:16714300).This antibody is specific to HECTD1.

实验方案

Product Specific Protocols
WB protocol for HECTD1 antibody 84918-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols