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验证数据展示

  • WB analysis using 22560-1-AP

    WB analysis using 22560-1-AP

    MCF-7 cells lysate was subjected to SDS PAGE followed by western blot with 22560-1-AP (HEATR6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 22560-1-AP

    IP experiment of MCF-7 using 22560-1-AP

    IP result of anti-HEATR6 (IP:22560-1-AP, 4 ug; Detection:22560-1-AP 1:1000) with MCF-7 cells lysate 1520 ug.

HEATR6 Polyclonal antibody

HEATR6 Polyclonal Antibody for WB, IP, ELISA
Cat No. 22560-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

ABC1, HEAT repeat containing 6, HEAT repeat-containing protein 6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IP detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22560-1-AP targets HEATR6 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18437

Product name: Recombinant human HEATR6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 832-1181 aa of BC119813

Sequence: AATSRALGVYVLFPCLRQDVIFVADAANAILMSLEDKSLNVRAKAAWSLGNLTDTLIVNMETPDPSFQEEFSGLLLLKMLRSAIEASKDKDKVKSNAVRALGNLLHFLQPSHIEKPTFAEIIEESIQALISTVLTEAAMKVRWNACYAMGNVFKNPALPLGTAPWTSQAYNALTSVVTSCKNFKVRIRSAAALSVPGKREQYGSVDQYARIWNALVTALQKSEDTIDFLEFKYCVSLRTQICQALIHLLSLASASDLPCMKETLELSGNMVQSYILQFLKSGAEGDDTGAPHSPQERDQMVRMALKHMGSIQAPTGDTARRAIMGFLEEILAVCFDSSGSQGALPGLTNQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HEAT repeat containing 6
别名 ABC1, HEAT repeat containing 6, HEAT repeat-containing protein 6
计算分子量 1181 aa, 129 kDa
观测分子量129 kDa
GenBank蛋白编号BC119813
基因名称 HEATR6
Gene ID (NCBI) 63897
RRIDAB_3742072
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ6AI08
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HEAT repeat-containing protein 6 (HEATR6) is amplified in certain breast cancer cell lines, such as MCF-7 and BT-474, suggesting its role in cancer progression. Additionally, HEATR6 is implicated in the regulation of gene expression, particularly in the context of long non-coding RNAs (lncRNAs) associated with breast cancer (PMID: 30953646).

实验方案

Product Specific Protocols
IP protocol for HEATR6 antibody 22560-1-APDownload protocol
WB protocol for HEATR6 antibody 22560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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