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  • Featured Product
  • KD/KO Validated

HDLBP Polyclonal antibody

HDLBP Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15406-1-AP

Print datasheet

Synonyms

HBP, HDL binding protein, HDLBP, PRO2900, VGL, Vigilin

验证数据展示

  • WB analysis using 15406-1-AP

    WB analysis using 15406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15406-1-AP (HDLBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 15406-1-AP

    IF Staining of MCF-7 using 15406-1-AP

    Immunofluorescent analysis of MCF-7 cells, using HDLBP antibody 15406-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of Jurkat using 15406-1-AP

    IP experiment of Jurkat using 15406-1-AP

    IP result of anti-HDLBP (IP:15406-1-AP, 4ug; Detection:15406-1-AP 1:500) with Jurkat cells lysate 2400ug.

  • IHC staining of mouse stomach using 15406-1-AP

    IHC staining of mouse stomach using 15406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15406-1-AP (HDLBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 15406-1-AP

    IHC staining of mouse stomach using 15406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15406-1-AP (HDLBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells
Positive IP detected inJurkat cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15406-1-AP targets HDLBP in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HDLBP fusion protein Ag7666 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name high density lipoprotein binding protein
Synonyms HBP, HDL binding protein, HDLBP, PRO2900, VGL, Vigilin
Calculated Molecular Weight 141 kDa
Observed Molecular Weight 141 kDa
GenBank Accession NumberBC001179
Gene Symbol HDLBP
Gene ID (NCBI) 3069
RRIDAB_2117367
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00341
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HDLBP antibody 15406-1-APDownload protocol
IHC protocol for HDLBP antibody 15406-1-APDownload protocol
IF protocol for HDLBP antibody 15406-1-APDownload protocol
IP protocol for HDLBP antibody 15406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Exp Cell Res

A new small cell lung cancer biomarker identified by Cell-SELEX generated aptamers.

Authors - Wei Zhou
  • KD Validated
View Article
humanWB

J Med Virol

Proteomic analysis of cell lines expressing small hepatitis B surface antigen revealed decreased glucose-regulated protein 78 kDa expression in association with higher susceptibility to apoptosis.

Authors - Zhao Chao C
View Article
humanWB,IHC,IF,CoIP

Cell Mol Gastroenterol Hepatol

HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation

Authors - Jingsheng Yuan
View Article