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验证数据展示

  • WB analysis of mouse liver using 26147-1-AP

    WB analysis of mouse liver using 26147-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26147-1-AP (HDHD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26147-1-AP

    IF Staining of HeLa using 26147-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDHD2 antibody (26147-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

HDHD2 Polyclonal antibody

HDHD2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26147-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Haloacid dehalogenase-like hydrolase domain-containing protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26147-1-AP targets HDHD2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag23518

Product name: Recombinant human HDHD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-169 aa of BC033031

Sequence: MLLVDDRALPDFKGIQTSDPNAVVMGLAPEHFHYQILNQAFRLLLDGAPLIAIHKARYYKRKDGLALGPGPFVTALEYATDTKATVVGKPEKTFFLEALRGTGCEPEEAVMIGDDCRDDVGGAQDVGMLGILVKTGKYRASDEEKINPPPYLTCESFPHAVDHILQHLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 haloacid dehalogenase-like hydrolase domain containing 2
别名 Haloacid dehalogenase-like hydrolase domain-containing protein 2
观测分子量 18 kDa, 28 kDa
GenBank蛋白编号BC033031
基因名称 HDHD2
Gene ID (NCBI) 84064
RRIDAB_2880404
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0R4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HDHD2 antibody 26147-1-APDownload protocol
IF protocol for HDHD2 antibody 26147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols