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HDHD2 Polyclonal antibody

HDHD2 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26147-1-AP

Print datasheet

Synonyms

3110052N05Rik, HDHD2

验证数据展示

  • WB analysis of mouse liver using 26147-1-AP

    WB analysis of mouse liver using 26147-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26147-1-AP (HDHD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26147-1-AP

    IF Staining of HepG2 using 26147-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 26147-1-AP (HDHD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26147-1-AP is HDHD2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse
Immunogen HDHD2 fusion protein Ag23518 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name haloacid dehalogenase-like hydrolase domain containing 2
Synonyms 3110052N05Rik, HDHD2
Observed Molecular Weight 18 kDa, 28 kDa
GenBank Accession NumberBC033031
Gene Symbol HDHD2
Gene ID (NCBI) 84064
RRIDAB_2880404
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0R4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HDHD2 antibody 26147-1-APDownload protocol
IF protocol for HDHD2 antibody 26147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols