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验证数据展示

  • WB analysis using 33953-1-AP

    WB analysis using 33953-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33953-1-AP (HDHD1A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of Caco-2 using 33953-1-AP

    IF Staining of Caco-2 using 33953-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using HDHD1A antibody (33953-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

HDHD1A Polyclonal antibody

HDHD1A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 33953-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

5'-PsiMPase, DXF68S1E, EC:3.1.3.96, FAM16AX, GS1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRamos cells, A549 cells, Caco-2 cells, Calu-1 cells, Raji cells
Positive IF/ICC detected inCaco-2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33953-1-AP targets HDHD1A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag40679

Product name: Recombinant human HDHD1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-214 aa of BC012494

Sequence: MDGLLLDTERLYSVVFQEICNRYDKKYSWDVKSLVMGKKALEAAQIIIDVLQLPMSKEELVEESQTKLKEVFPTAALMPGAEKLIIHLRKHGIPFALATSSRSASFDMKTSRHKEFFSLFSHIVLGDDPEVQHGKPDPDIFLACAKRFSPPPAMEKCLVFEDAPNGVEAALAAGMQVVMVPDGNLSRDLTTKATLVLNSLQDFQPELFGLPSYE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 haloacid dehalogenase-like hydrolase domain containing 1A
别名 5'-PsiMPase, DXF68S1E, EC:3.1.3.96, FAM16AX, GS1
计算分子量 228 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC012494
基因名称 HDHD1A
Gene ID (NCBI) 8226
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ08623
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The enzyme encoded by human HDHD1A (hHDHD1A; also known as HDHD1, PUDP, GS1; Gene ID: 8226) has activity specific for ΨMP 5'-phosphatase (PMID: 20722631). HDHD1A belongs to the HAD-like hydrolase superfamily. In humans, the enzyme HDHD1A dephosphorylates pseudouridine 5'-monophosphate (ΨMP), and the resulting Ψ is excreted in the urine (PMID: 40409548).

实验方案

Product Specific Protocols
IF protocol for HDHD1A antibody 33953-1-APDownload protocol
WB protocol for HDHD1A antibody 33953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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