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验证数据展示

  • WB analysis using 12380-1-AP (same clone as 12380-1-PBS)

    WB analysis using 12380-1-AP (same clone as 12380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12380-1-AP (HDGFRP3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 12380-1-AP (same clone as 12380-1-PBS)

    IHC staining of human prostate cancer using 12380-1-AP (same clone as 12380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12380-1-AP (HDGFRP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 12380-1-AP (same clone as 12380-1-PBS)

    IP experiment of mouse testis using 12380-1-AP (same clone as 12380-1-PBS)

    IP result of anti-HDGFRP3 (IP:12380-1-AP, 3ug; Detection:12380-1-AP 1:300) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 12380-1-AP (same clone as 12380-1-PBS)

    IF Staining of MCF-7 using 12380-1-AP (same clone as 12380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HDGFRP3 antibody (12380-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HDGFRP3 Polyclonal antibody, PBS Only

HDGFRP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12380-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

HDGF-2, HDGF2, HDGF 2, CGI-142, CGI142

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12380-1-PBS targets HDGFRP3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3046

Product name: Recombinant human HDGFRP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-203 aa of BC015483

Sequence: MARPRPREYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQAIQQQSSSETEGEGGNTADASSEEEGDRVEEDGKGKRKNEKAGSKRKKSYTSKKSSKQSRKSPGDEDDKDCKEEENKSSSEGGDAGNDTRNTTSDLQKTSEGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hepatoma-derived growth factor, related protein 3
别名 HDGF-2, HDGF2, HDGF 2, CGI-142, CGI142
计算分子量 203 aa, 23 kDa
观测分子量 30 kDa
GenBank蛋白编号BC015483
基因名称 HDGFRP3
Gene ID (NCBI) 50810
RRIDAB_2117222
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3E1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HDGFRP3, also named as Hepatoma-derived growth factor-related protein 3 or HDGF2, is a 203 amino acid protein, which contains 1 PWWP domain and belongs to the HDGF family. HDGFRP3 detected in testis, heart, spinal cord and brain. HDGFRP3 as a nuclear potein enhances DNA synthesis and may play a role in cell proliferation. HDGFRP3 is a 30 kDa protein for phosphor modification.

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