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验证数据展示

  • WB analysis of SMMC-7721 using 60064-1-Ig (same clone as 60064-1-PBS)

    WB analysis of SMMC-7721 using 60064-1-Ig (same clone as 60064-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

    IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDGF antibody included
  • IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

    IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDGF antibody included

HDGF Monoclonal antibody, PBS Only

HDGF Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60064-1-PBS

产品说明书

CloneNo. 5A7G3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

5A7G3, Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HDGF Ready-To-Use IHC Kit. HDGF primary antibody included.

产品信息

60064-1-PBS targets HDGF in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1893

Product name: Recombinant human HDGF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-240 aa of BC018991

Sequence: MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hepatoma-derived growth factor (high-mobility group protein 1-like)
别名 5A7G3, Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2
计算分子量 26 kDa
观测分子量 40 kDa
GenBank蛋白编号BC018991
基因名称 HDGF
Gene ID (NCBI) 3068
RRIDAB_2117098
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP51858
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)

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