验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, human heart tissue, mouse heart tissue, mouse kidney tissue, mouse lung tissue, mouse pancreas tissue, mouse testis tissue, A549 cells, rat heart tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue, human brain tissue, human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
21091-1-AP targets HDDC3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | HDDC3 fusion protein Ag15342 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | HD domain containing 3 |
| 别名 | HD domain containing 3, HD domain containing protein 3, HDDC3, MESH1 |
| 计算分子量 | 179 aa, 20 kDa |
| 观测分子量 | 22-25 kDa |
| GenBank蛋白编号 | BC033794 |
| 基因名称 | HDDC3 |
| Gene ID (NCBI) | 374659 |
| RRID | AB_10733121 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N4P3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MESH1, encodes by HDDC3 in human and Q9VAM9 in Drosophila melanogaster, was identified as functional SpoT orthologs in metazoan (PMID: 20818390). MESH1 is a cytosolic NADPH phosphatase that is induced under stress conditions, leading to the NADPH depletion and ferroptosis-a novel form of iron-dependent regulated cell death characterized by lipid peroxidation. Accordingly, MESH1 removal preserves the NADPH level in stressed cells and promotes their ferroptotic survival(PMID: 32462112, PMID: 34294679).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HDDC3 antibody 21091-1-AP | Download protocol |
| IHC protocol for HDDC3 antibody 21091-1-AP | Download protocol |
| IP protocol for HDDC3 antibody 21091-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomolecules Molecular Profiling of Mouse Models of Loss or Gain of Function of the KCNT1 (Slack) Potassium Channel and Antisense Oligonucleotide Treatment |
