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验证数据展示

  • WB analysis of HeLa using 68391-1-Ig

    WB analysis of HeLa using 68391-1-Ig

    WB result of HDDC2 antibody (68391-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDDC2 transfected HeLa cells.

  • IHC staining of mouse kidney using 68391-1-Ig

    IHC staining of mouse kidney using 68391-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68391-1-Ig (HDDC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDDC2 antibody included
  • WB analysis using 68391-1-Ig

    WB analysis using 68391-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68391-1-Ig (HDDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Saos-2 using 68391-1-Ig

    IF Staining of Saos-2 using 68391-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using HDDC2 antibody (68391-1-Ig, Clone: 2E1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of U2OS using 68391-1-Ig

    FC experiment of U2OS using 68391-1-Ig

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug HDDC2 Monoclonal antibody (68391-1-Ig, Clone:2E1B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

HDDC2 Monoclonal antibody

HDDC2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 68391-1-Ig

产品说明书

CloneNo. 2E1B2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

EC:3.1.3.89, CGI-130, CGI 130, C6orf74, 5'-deoxynucleotidase HDDC2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells
Positive IHC detected inmouse kidney tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSaos-2 cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy HDDC2 Ready-To-Use IHC Kit. HDDC2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68391-1-Ig targets HDDC2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HDDC2 fusion protein Ag33258 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 HD domain containing 2
别名 EC:3.1.3.89, CGI-130, CGI 130, C6orf74, 5'-deoxynucleotidase HDDC2
计算分子量 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC003357
基因名称 HDDC2
Gene ID (NCBI) 51020
RRIDAB_3085109
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z4H3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDDC2, also named as C6orf74, plays a role in the maintenance of pluripotency in human ES and iPS cells. HDDC2 is a nuclear‐encoded but mitochondrially localized protein. It has been reported as associated with oxidative metabolism.

实验方案

Product Specific Protocols
WB protocol for HDDC2 antibody 68391-1-IgDownload protocol
IHC protocol for HDDC2 antibody 68391-1-IgDownload protocol
IF protocol for HDDC2 antibody 68391-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols