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验证数据展示

  • WB analysis using 16632-1-AP

    WB analysis using 16632-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16632-1-AP (HDDC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16632-1-AP

    WB analysis of HeLa using 16632-1-AP

    WB result of HDDC2 antibody (16632-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDDC2 transfected HeLa cells.

  • IHC staining of human brain using 16632-1-AP

    IHC staining of human brain using 16632-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16632-1-AP (HDDC2 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with HDDC2 antibody included
  • IHC staining of human brain using 16632-1-AP

    IHC staining of human brain using 16632-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16632-1-AP (HDDC2 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with HDDC2 antibody included
  • IF Staining of PC-3 using 16632-1-AP

    IF Staining of PC-3 using 16632-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 16632-1-AP (HDDC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

HDDC2 Polyclonal antibody

HDDC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16632-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

5'-deoxynucleotidase HDDC2, C6orf74, CGI 130, CGI-130, EC:3.1.3.89

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, PC-3 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy HDDC2 Ready-To-Use IHC Kit. HDDC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16632-1-AP targets HDDC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9971

Product name: Recombinant human HDDC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 15-218 aa of BC003357

Sequence: MASVSSATFSGHGARSLLQFLRLVGQLKRVPRTGWVYRNVQRPESVSDHMYRMAVMAMVIKDDRLNKDRCVRLALVHDMAECIVGDIAPADNIPKEEKHRREEEAMKQITQLLPEDLRKELYELWEEYETQSSAEAKFVKQLDQCEMILQASEYEDLEHKPGRLQDFYDSTAGKFNHPEIVQLVSELEAERSTNIAAAASEPHS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HD domain containing 2
别名 5'-deoxynucleotidase HDDC2, C6orf74, CGI 130, CGI-130, EC:3.1.3.89
计算分子量 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC003357
基因名称 HDDC2
Gene ID (NCBI) 51020
RRIDAB_10597248
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z4H3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDDC2, also named as C6orf74, plays a role in the maintenance of pluripotency in human ES and iPS cells. HDDC2 is a nuclear‐encoded but mitochondrially localized protein. It has been reported as associated with oxidative metabolism.

实验方案

Product Specific Protocols
IF protocol for HDDC2 antibody 16632-1-APDownload protocol
IHC protocol for HDDC2 antibody 16632-1-APDownload protocol
WB protocol for HDDC2 antibody 16632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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