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HDDC2 Polyclonal antibody

HDDC2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16632-1-AP

Print datasheet

Synonyms

C6orf74, CGI 130, HD domain containing 2, HD domain containing protein 2, HDDC2, NS5ATP2

验证数据展示

  • WB analysis using 16632-1-AP

    WB analysis using 16632-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16632-1-AP (HDDC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16632-1-AP

    IHC staining of human brain using 16632-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16632-1-AP (HDDC2 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with HDDC2 antibody included
  • IHC staining of human brain using 16632-1-AP

    IHC staining of human brain using 16632-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16632-1-AP (HDDC2 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with HDDC2 antibody included
  • IF Staining of PC-3 using 16632-1-AP

    IF Staining of PC-3 using 16632-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 16632-1-AP (HDDC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, PC-3 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy HDDC2 Ready-To-Use IHC Kit. HDDC2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16632-1-AP targets HDDC2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen HDDC2 fusion protein Ag9971 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HD domain containing 2
Synonyms C6orf74, CGI 130, HD domain containing 2, HD domain containing protein 2, HDDC2, NS5ATP2
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC003357
Gene Symbol HDDC2
Gene ID (NCBI) 51020
RRIDAB_10597248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4H3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDDC2, also named as C6orf74, plays a role in the maintenance of pluripotency in human ES and iPS cells. HDDC2 is a nuclear‐encoded but mitochondrially localized protein. It has been reported as associated with oxidative metabolism.

实验方案

Product Specific Protocols
WB protocol for HDDC2 antibody 16632-1-APDownload protocol
IHC protocol for HDDC2 antibody 16632-1-APDownload protocol
IF protocol for HDDC2 antibody 16632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols