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HDAC8 Polyclonal antibody

HDAC8 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17548-1-AP

Print datasheet

Synonyms

HD8, HDAC8, HDACL1, histone deacetylase 8, RPD3



经过测试的应用

Positive WB detected inHeLa cells, Raji cells
Positive IP detected inRaji cells
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HDAC8 Ready-To-Use IHC Kit. HDAC8 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17548-1-AP targets HDAC8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IP, WB,ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen HDAC8 fusion protein Ag11696 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone deacetylase 8
Synonyms HD8, HDAC8, HDACL1, histone deacetylase 8, RPD3
Calculated Molecular Weight 377 aa, 42 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC050433
Gene Symbol HDAC8
Gene ID (NCBI) 55869
RRIDAB_2116926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY41
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.

实验方案

Product Specific Protocols
WB protocol for HDAC8 antibody 17548-1-APDownload protocol
IF protocol for HDAC8 antibody 17548-1-APDownload protocol
IP protocol for HDAC8 antibody 17548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.

Authors - Xun Huang
ratWB

Cell Rep

Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.

Authors - Tian Wang
mouse, ratWB

Kidney Int

Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.

Authors - Xiaojie Wang
humanWB

Oncotarget

Thrombospondin-1 might be a therapeutic target to suppress RB cells by regulating the DNA double-strand breaks repair.

Authors - Pei Chen
humanWB

Oncogenesis

USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.

Authors - Wei Zhan
humanWB

Molecules

Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway

Authors - Xintian Lan