• Featured Product
  • KD/KO Validated

HDAC6 Polyclonal antibody

HDAC6 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 12834-1-AP

Print datasheet

Synonyms

HD6, HDAC6, histone deacetylase 6, JM21, KIAA0901



经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, human liver tissue, HepG2 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman testis tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, MG132 treated HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HDAC6 Ready-To-Use IHC Kit. HDAC6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12834-1-AP targets HDAC6 in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA Application Description
Cited ApplicationsWB, IP, IF, RIP, IHC, CoIP
Tested Reactivity human
Cited Reactivityhuman, canine
Immunogen HDAC6 fusion protein Ag3572 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone deacetylase 6
Synonyms HD6, HDAC6, histone deacetylase 6, JM21, KIAA0901
Calculated Molecular Weight 1063 aa, 114 kDa, 131 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC013737
Gene Symbol HDAC6
Gene ID (NCBI) 10013
RRIDAB_10597094
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBN7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is a rabbit polyclonal antibody raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.

实验方案

Product Specific Protocols
WB protocol for HDAC6 antibody 12834-1-APDownload protocol
IHC protocol for HDAC6 antibody 12834-1-APDownload protocol
IF protocol for HDAC6 antibody 12834-1-APDownload protocol
IP protocol for HDAC6 antibody 12834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF,WB

Bioact Mater

Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway

Authors - Tian Qin
humanWB

Mol Cell

An Interaction Landscape of Ubiquitin Signaling.

Authors - Xiaofei Zhang
humanWB,IP

Genome Biol

The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots

Authors - Shenli Yuan
humanWB,IP

Oncogene

HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer

Authors - Bei Liu
  • KD Validated
WB

Kidney Int

Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.

Authors - Xiaojie Wang
humanWB,IHC

Biomed Pharmacother

Degradation of histone deacetylase 6 alleviates ROS-mediated apoptosis in renal ischemia-reperfusion injury

Authors - Kang Xia