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HDAC3 Polyclonal antibody

HDAC3 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IF/ICC, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10255-1-AP

Print datasheet

Synonyms

HD3, HDAC3, histone deacetylase 3, RPD3, RPD3 2, SMAP45



经过测试的应用

Positive WB detected inA431 cells, HL-60 cells, HeLa cells, U-251 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy HDAC3 Ready-To-Use IHC Kit. HDAC3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10255-1-AP targets HDAC3 in WB, IP, IF, IHC, CoIP, chIP, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC, CoIP, chIP
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen HDAC3 fusion protein Ag0396 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone deacetylase 3
Synonyms HD3, HDAC3, histone deacetylase 3, RPD3, RPD3 2, SMAP45
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC000614
Gene Symbol HDAC3
Gene ID (NCBI) 8841
RRIDAB_2279733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15379
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of HDAC3? Is HDAC3 post-translationally modified?

The molecular weight of HDAC3 is 49 kDa but HDAC3 can form homodimers and homotrimers in cells (PMID: 11779848). HDAC3 can be phosphorylated at S424 residue by protein kinase CK2, which regulates HDAC3 activity (PMID: 15805470).

 What is the subcellular localization of HDAC3?

Unlike other class I HDACs, HDAC3 actively shuttles between the nucleus and cytoplasm (PMID: 11779848). Additionally, HDAC3 can be present at the plasma membrane, where it associates with c-Src (PMID: 16532030).

 What is the tissue expression pattern of HDAC3?

Like other class I HDACs, HDAC3 is ubiquitously expressed (PMID: 9346952).

 What is the mechanism of HDAC3 in regulating gene expression?

Unlike other histone deacetylates, it exists in a complex with two proteins - nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) - and therefore acts as a mediator of the repression function of unliganded nuclear hormone receptors (PMID: 10809664). N-CoR and SMRT play an active role in the recruitment of HDAC3 to binding sites as well as directly regulating HDAC3 activity. Additionally, HDAC3 targets growth-regulated genes.


实验方案

Product Specific Protocols
WB protocol for HDAC3 antibody 10255-1-APDownload protocol
IHC protocol for HDAC3 antibody 10255-1-APDownload protocol
IF protocol for HDAC3 antibody 10255-1-APDownload protocol
IP protocol for HDAC3 antibody 10255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Acta Pharm Sin B

Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.

Authors - Xin Sun
mouseWB

J Clin Invest

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

Authors - Shanshan Zhong
humanWB

Mol Ther

Genetic and Pharmacological Inhibition of GRPR Protects Against Acute Kidney Injury via Attenuating Renal Inflammation and Necroptosis

Authors - Chao Li
mouseWB

J Exp Med

Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.

Authors - Zhe Chen
ratWB

Cell Rep

Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.

Authors - Tian Wang

Oncogene

miR-155 downregulates ErbB2 and suppresses ErbB2-induced malignant transformation of breast epithelial cells.

Authors - X-H He