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验证数据展示

  • WB analysis using 67646-1-Ig (same clone as 67646-1-PBS)

    WB analysis using 67646-1-Ig (same clone as 67646-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

  • WB analysis using 67646-1-Ig (same clone as 67646-1-PBS)

    WB analysis using 67646-1-Ig (same clone as 67646-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 67646-1-Ig (same clone as 67646-1-PBS)

    IF Staining of Jurkat using 67646-1-Ig (same clone as 67646-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67646-1-Ig (same clone as 67646-1-PBS)

    IF Staining of MCF-7 using 67646-1-Ig (same clone as 67646-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

HDAC10 Monoclonal antibody, PBS Only

HDAC10 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67646-1-PBS

产品说明书

CloneNo. 1A1B11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

Polyamine deacetylase HDAC10, HDAC 10, HD10, HD 10, EC:3.5.1.48

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HDAC10 Ready-To-Use IHC Kit. HDAC10 primary antibody included.

产品信息

67646-1-PBS targets HDAC10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HDAC10 fusion protein Ag18470 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 histone deacetylase 10
别名 Polyamine deacetylase HDAC10, HDAC 10, HD10, HD 10, EC:3.5.1.48
计算分子量 669 aa, 71 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC125083
基因名称 HDAC10
Gene ID (NCBI) 83933
RRIDAB_2882846
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ969S8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.