HDAC1 Monoclonal antibody

HDAC1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat


IF/ICC, IHC, WB, ELISA and More (1)





Cat No : 66085-1-Ig

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GON 10, HD1, HDAC, HDAC1, histone deacetylase 1, RPD3, RPD3L1


Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman lymphoma tissue, human lung cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy HDAC1 Ready-To-Use IHC Kit. HDAC1 primary antibody included.


Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:6000-1:24000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


66085-1-Ig targets HDAC1 in WB, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA Application Description
Cited ApplicationsWB, IHC, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen HDAC1 fusion protein Ag19128 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name histone deacetylase 1
Synonyms GON 10, HD1, HDAC, HDAC1, histone deacetylase 1, RPD3, RPD3L1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC000301
Gene Symbol HDAC1
Gene ID (NCBI) 3065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .


Product Specific Protocols
WB protocol for HDAC1 antibody 66085-1-IgDownload protocol
IHC protocol for HDAC1 antibody 66085-1-IgDownload protocol
IF protocol for HDAC1 antibody 66085-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner.

Authors - Moumita Datta

J Clin Invest

The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.

Authors - Wanjin Li

Nat Commun

ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin.

Authors - Lifang Li

Br J Pharmacol

Physalin B attenuates liver fibrosis via suppressing LAP2α-HDAC1-mediated deacetylation of the transcription factor GLI1 and hepatic stellate cell activation.

Authors - Xiaoyun Zhu

Front Cell Dev Biol

LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6.

Authors - Huihua Kai


Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway

Authors - Xintian Lan