验证数据展示
经过测试的应用
| Positive WB detected in | C6 cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32859-1-AP targets HCRTR2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag38128 Product name: Recombinant human HCRTR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 380-444 aa of NM_001526 Sequence: SCCCLGVHHRQEDRLTRGRTSTESRKSLTTQISNFDNISKLSEQVVLTSISTLPAANGAGPLQNW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hypocretin (orexin) receptor 2 |
| 别名 | Hypocretin receptor type 2, Orexin receptor type 2, Ox2R, Ox-2-R, Ox2-R |
| 计算分子量 | 440aa, 51 kDa |
| 观测分子量 | 70-73 kDa |
| GenBank蛋白编号 | NM_001526 |
| 基因名称 | HCRTR2 |
| Gene ID (NCBI) | 3062 |
| RRID | AB_3742766 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O43614 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HCRTR2 encodes hypocretin receptor 2, a G protein-coupled receptor that binds the neuropeptides orexin-A and orexin-B with high affinity. Through this binding, it activates G(q)/11 and G(i)/o signaling to modulate cytoplasmic Ca2+ levels and neuronal excitability, placing it at the center of sleep-wake cycle regulation and central glucose homeostasis. Protein expression is high in the brain, consistent with its role in neural control of arousal and metabolism.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HCRTR2 antibody 32859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
