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验证数据展示

  • WB analysis using 30946-1-AP (same clone as 30946-1-PBS)

    WB analysis using 30946-1-AP (same clone as 30946-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30946-1-AP (HCN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 30946-1-AP (same clone as 30946-1-PBS)

    IHC staining of mouse cerebellum using 30946-1-AP (same clone as 30946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 30946-1-AP (same clone as 30946-1-PBS)

    IHC staining of mouse brain using 30946-1-AP (same clone as 30946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 30946-1-AP (same clone as 30946-1-PBS)

    IF Staining of mouse brain using 30946-1-AP (same clone as 30946-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using HCN2 antibody (30946-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

HCN2 Polyclonal antibody, PBS Only

HCN2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30946-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

BCNG2, BCNG-2, Brain cyclic nucleotide-gated channel 2, HAC 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30946-1-PBS targets HCN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34038

Product name: Recombinant human HCN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 750-889 aa of NM_001194

Sequence: LALGSPRLVRRPPPGPAPAAASPGPPPPASPPGAPASPRAPRTSPYGGLPAAPLAGPALPARRLSRASRPLSASQPSLPHGAPGPAASTRPASSSTPRLGPTPAARAAAPSPDRRDSASPGAAGGLDPQDSARSRLSSNL*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hyperpolarization activated cyclic nucleotide-gated potassium channel 2
别名 BCNG2, BCNG-2, Brain cyclic nucleotide-gated channel 2, HAC 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
计算分子量 97 kDa
观测分子量60 kDa, 90 kDa, 120 kDa
GenBank蛋白编号NM_001194
基因名称 HCN2
Gene ID (NCBI) 610
RRIDAB_3086427
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UL51
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. The molecular weight of immature HCN2 is 90 kDa, and the protein is about 120 kDa after n-terminal glycosylation. It has been found that 60 kDa of HCN2 protein can be identified in mouse heart samples. Although all protein isolation steps were handled on ice and in the presence of protease inhibitors to reduce protease-dependent effects and to minimize protein degradation, it is certainly possible that the presence of the 60 kDa HCN2 protein in the heart samples does, in fact, reflect protein degradation. (PMID:21796099, PMID: 19574228)

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