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验证数据展示

  • WB analysis using 84845-3-RR

    WB analysis using 84845-3-RR

    Boiled and unboiled mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 84845-3-RR (HCN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 84845-3-RR

    IHC staining of mouse cerebellum using 84845-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84845-3-RR (HCN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HCN1 Recombinant antibody

HCN1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84845-3-RR

产品说明书

CloneNo. 242254A1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, HAC 2, Brain cyclic nucleotide-gated channel 1, BCNG-1, 242254A1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84845-3-RR targets HCN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hyperpolarization activated cyclic nucleotide-gated potassium channel 1
别名 Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, HAC 2, Brain cyclic nucleotide-gated channel 1, BCNG-1, 242254A1
计算分子量 99 kDa
观测分子量 99-120 kDa
GenBank蛋白编号NM_021072
基因名称 HCN1
Gene ID (NCBI) 348980
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60741
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HCN1, also named as BCNG1, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. HCN1 contributes to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. HCN1 may mediate responses to sour stimuli.

实验方案

Product Specific Protocols
WB protocol for HCN1 antibody 84845-3-RRDownload protocol
IHC protocol for HCN1 antibody 84845-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols