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HCCS Polyclonal antibody

HCCS Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15118-1-AP

Print datasheet

Synonyms

CCHL, Cytochrome c type heme lyase, HCCS, Holocytochrome c type synthase, MCOPS7

验证数据展示

  • WB analysis of human heart using 15118-1-AP

    WB analysis of human heart using 15118-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15118-1-AP (HCCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 15118-1-AP

    IP experiment of MCF-7 using 15118-1-AP

    IP result of anti-HCCS (IP:15118-1-AP, 4ug; Detection:15118-1-AP 1:500) with MCF-7 cells lysate 3200ug.

  • IHC staining of human breast cancer using 15118-1-AP

    IHC staining of human breast cancer using 15118-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 15118-1-AP

    IHC staining of human breast cancer using 15118-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15118-1-AP

    IF Staining of HeLa using 15118-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15118-1-AP (HCCS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman heart tissue, HeLa cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

15118-1-AP targets HCCS in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen HCCS fusion protein Ag7189 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name holocytochrome c synthase (cytochrome c heme-lyase)
Synonyms CCHL, Cytochrome c type heme lyase, HCCS, Holocytochrome c type synthase, MCOPS7
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC001691
Gene Symbol HCCS
Gene ID (NCBI) 3052
RRIDAB_2114848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53701
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HCCS(Cytochrome c-type heme lyase) links covalently the heme group to the apoprotein of cytochrome C.The covalent attachment of heme by CCHL in the IMS is crucial for translocation of the precursor of cytochrome c and its trapping in the IMS(PMID:15359280 ).This protein has a calculated molecular mass of about 31 KDa.

实验方案

Product Specific Protocols
WB protocol for HCCS antibody 15118-1-APDownload protocol
IHC protocol for HCCS antibody 15118-1-APDownload protocol
IF protocol for HCCS antibody 15118-1-APDownload protocol
IP protocol for HCCS antibody 15118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Mol Med

SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.

Authors - Alexandre Janer
View Article
humanWB

Chem Pharm Bull (Tokyo)

The Novel Synthetic Triterpene Methyl 3β-O-[4-(2-Aminoethylamino)-4-oxo-butyryl]olean-12-ene-28-oate Inhibits Breast Tumor Cell Growth in Vitro and in Vivo.

Authors - Bin Feng
View Article
human,mouseWB

Nat Cell Biol

Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space

Authors - Elias Adriaenssens
View Article