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验证数据展示

  • WB analysis of K-562 using 83576-4-RR (same clone as 83576-4-PBS)

    WB analysis of K-562 using 83576-4-RR (same clone as 83576-4-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 83576-4-RR (HBQ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83576-4-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 83576-4-RR (same clone as 83576-4-PBS)

    IF Staining of K-562 using 83576-4-RR (same clone as 83576-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HBQ1 antibody (83576-4-RR, Clone: 240527D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83576-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83576-4-PBS

    Affinity and Kinetic Characterization of 83576-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 83576-4-PBS against Human HBQ1 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83576-4-RR

    Affinity and Kinetic Characterization of 83576-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83576-4-RR against Human HBQ1 were performed. The affinity constant is 73.4 pM.

HBQ1 Recombinant monoclonal antibody, PBS Only

HBQ1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83576-4-PBS

产品说明书

CloneNo. 240527D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

240527D6, Hemoglobin subunit theta 1, Hemoglobin subunit theta-1, Hemoglobin theta 1 chain, Hemoglobin theta-1 chain

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83576-4-PBS targets HBQ1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10105

Product name: Recombinant human HBQ1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-142 aa of BC056686

Sequence: MALSAEDRALVRALWKKLGSNVGVYTTEALERTFLAFPATKTYFSHLDLSPGSSQVRAHGQKVADALSLAVERLDDLPHALSALSHLHACQLRVDPASFQLLGHCLLVTLARHYPGDFSPALQASLDKFLSHVISALVSEYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hemoglobin, theta 1
别名 240527D6, Hemoglobin subunit theta 1, Hemoglobin subunit theta-1, Hemoglobin theta 1 chain, Hemoglobin theta-1 chain
计算分子量 142 aa, 16 kDa
观测分子量10~16 kDa
GenBank蛋白编号BC056686
基因名称 HBQ1
Gene ID (NCBI) 3049
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP09105
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HBQ1 (Hemoglobin subunit theta-1) is a member of the human alpha-globin gene cluster. HBQ1 mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue (PMID: 3657976). HBQ1 gene was transcribed in an erythroleukemia cell line. HBQ1 has transcriptionally active and may be expressed in early erythroid tissue (PMID: 3422341).

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