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验证数据展示

  • WB analysis using 16706-1-AP

    WB analysis using 16706-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16706-1-AP (HBQ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 16706-1-AP

    IHC staining of human placenta using 16706-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16706-1-AP (HBQ1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human placenta using 16706-1-AP

    IHC staining of human placenta using 16706-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16706-1-AP (HBQ1 antibody) at dilution of 1:200 (under 40x lens).

HBQ1 Polyclonal antibody

HBQ1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16706-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Theta 1 globin, hemoglobin, theta 1, Hemoglobin theta-1 chain, Hemoglobin theta 1 chain, Hemoglobin subunit theta-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16706-1-AP targets HBQ1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10105

Product name: Recombinant human HBQ1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-142 aa of BC056686

Sequence: MALSAEDRALVRALWKKLGSNVGVYTTEALERTFLAFPATKTYFSHLDLSPGSSQVRAHGQKVADALSLAVERLDDLPHALSALSHLHACQLRVDPASFQLLGHCLLVTLARHYPGDFSPALQASLDKFLSHVISALVSEYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemoglobin, theta 1
别名 Theta 1 globin, hemoglobin, theta 1, Hemoglobin theta-1 chain, Hemoglobin theta 1 chain, Hemoglobin subunit theta-1
计算分子量 142 aa, 16 kDa
观测分子量10~16 kDa
GenBank蛋白编号BC056686
基因名称 HBQ1
Gene ID (NCBI) 3049
RRIDAB_1851212
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09105
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HBQ1 (Hemoglobin subunit theta-1) is a member of the human alpha-globin gene cluster. HBQ1 mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue (PMID: 3657976). HBQ1 gene was transcribed in an erythroleukemia cell line. HBQ1 has transcriptionally active and may be expressed in early erythroid tissue (PMID: 3422341).

实验方案

Product Specific Protocols
IHC protocol for HBQ1 antibody 16706-1-APDownload protocol
WB protocol for HBQ1 antibody 16706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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