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验证数据展示

  • WB analysis using 31753-1-AP

    WB analysis using 31753-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31753-1-AP (HBG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 31753-1-AP

    WB analysis using 31753-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31753-1-AP (HBG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HBG2 Polyclonal antibody

HBG2 Polyclonal Antibody for WB, ELISA
Cat No. 31753-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

hemoglobin, gamma G, Hemoglobin subunit gamma 2, Hemoglobin gamma G chain, Hemoglobin gamma 2 chain, Hb F Ggamma

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, TF-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31753-1-AP targets HBG2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 HBG2 fusion protein Ag36028 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemoglobin, gamma G
别名 hemoglobin, gamma G, Hemoglobin subunit gamma 2, Hemoglobin gamma G chain, Hemoglobin gamma 2 chain, Hb F Ggamma
计算分子量 16 kDa
观测分子量14~16 kDa
GenBank蛋白编号BC029387
基因名称 HBG2
Gene ID (NCBI) 3048
RRIDAB_3670105
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP69892
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gamma globin genes (HBG2, encoding Gγ-globin; HBG1 encoding Aγ-globin) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. Gamma chain production continues into adulthood in some beta-thalassemias and related conditions.

实验方案

Product Specific Protocols
WB protocol for HBG2 antibody 31753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols