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验证数据展示

  • WB analysis using 86851-1-RR

    WB analysis using 86851-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86851-1-RR (HBG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 86851-1-RR

    IF Staining of K-562 using 86851-1-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HBG1 antibody (86851-1-RR, Clone: 251754H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86851-1-RR

    Affinity and Kinetic Characterization of 86851-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86851-1-RR against Human HBG1 were performed. The affinity constant is 0.941 nM.

HBG1 Recombinant monoclonal antibody

HBG1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86851-1-RR

产品说明书

CloneNo. 251754H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Gamma 1 globin, Gamma-1-globin, Hb F Agamma, HBGA, HBGR

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEL cells, K-562 cells
Positive IF/ICC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy HBG1 Ready-To-Use IHC Kit. HBG1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86851-1-RR targets HBG1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22776

Product name: Recombinant human HBG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-118 aa of BC010913

Sequence: MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPKVKAHGKKVLTSLGDATKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hemoglobin, gamma A
别名 Gamma 1 globin, Gamma-1-globin, Hb F Agamma, HBGA, HBGR
计算分子量 147 aa, 16 kDa
观测分子量 14-16 kDa
GenBank蛋白编号BC010913
基因名称 HBG1
Gene ID (NCBI) 3047
RRIDAB_3745163
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP69891
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HBG1, also named as Hb F Agamma, HBGA, HBGR and HSGGL, belongs to the globin family. HBG1 makes up the fetal hemoglobin F, in combination with alpha chains. Some gamma variants can cause severe jaundice and cyanosis in premature and new born babies. The antibody is specific to HBG1 and HBG2. The antibody has no cross reaction to HBB and HBD. HBG1 protein migrates around 16 kDa. Heterodimer of 32 kDa may also be observed. This antibody could identify HBG1 and HBG2.

实验方案

Product Specific Protocols
IF protocol for HBG1 antibody 86851-1-RRDownload protocol
WB protocol for HBG1 antibody 86851-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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