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验证数据展示

  • WB analysis of K-562 using 66151-1-Ig

    WB analysis of K-562 using 66151-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66151-1-Ig (HBE1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 66151-1-Ig

    IHC staining of human placenta using 66151-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66151-1-Ig (HBE1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HBE1-Specific Monoclonal antibody

HBE1-Specific Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66151-1-Ig

产品说明书

CloneNo. 2C11G6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

HBE1, Hemoglobin epsilon, 2C11G6, Epsilon globin, Epsilon-globin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66151-1-Ig targets HBE1-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hemoglobin, epsilon 1
别名 HBE1, Hemoglobin epsilon, 2C11G6, Epsilon globin, Epsilon-globin
观测分子量 16 kDa
GenBank蛋白编号NM_005330
基因名称 HBE1
Gene ID (NCBI) 3046
RRIDAB_2881547
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP02100
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hemoglobin molecule is a tetramer consisting of two α-globin-like polypeptide chains and two β-globin-like chains. The human hemoglobin genes are expressed in a tightly developmentally controlled fashion.ε-globin (HBE1) is the predominantly expressed gene during the embryonic stage. The epsilon hemoglobin chain seems to be the best marker for fetal nucleated red blood cells (NRBCs). Anti-HBE1 may be used to label and isolate fetal cells from maternal blood and can be useful in prenatal diagnosis. This antibody specifically recognizes the HBE1 and doesn't cross-react with other globin chains.

实验方案

Product Specific Protocols
FC protocol for HBE1-Specific antibody 66151-1-IgDownload protocol
IHC protocol for HBE1-Specific antibody 66151-1-IgDownload protocol
WB protocol for HBE1-Specific antibody 66151-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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