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HBD Polyclonal antibody

HBD Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16824-1-AP

Print datasheet

Synonyms

Delta globin, HBD, Hemoglobin delta chain, Hemoglobin subunit delta, hemoglobin, delta

验证数据展示

  • WB analysis of human plasma using 16824-1-AP

    WB analysis of human plasma using 16824-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 16824-1-AP (HBD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16824-1-AP

    IHC staining of human liver using 16824-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16824-1-AP (HBD antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16824-1-AP

    IHC staining of human liver using 16824-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16824-1-AP (HBD antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inhuman blood tissue, human plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16824-1-AP targets HBD in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HBD fusion protein Ag10409 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hemoglobin, delta
Synonyms Delta globin, HBD, Hemoglobin delta chain, Hemoglobin subunit delta, hemoglobin, delta
Calculated Molecular Weight 147 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC070282
Gene Symbol HBD
Gene ID (NCBI) 3045
RRIDAB_2114476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02042
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HBD antibody 16824-1-APDownload protocol
IHC protocol for HBD antibody 16824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Front Pharmacol

Beneficial Effect of Edoxaban on Preventing Atrial Fibrillation and Coagulation by Reducing Inflammation via HBG1/HBD Biomarkers.

Authors - Chenguang Yang
View Article
humanWB

Placenta

SWATH proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy

Authors - Yuxuan Jiang
View Article