验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, human placenta, human peripheral blood platelets, human plasma, rabbit brain, rabbit liver |
Planning an IHC experiment? We recommend our IHCeasy HBB Ready-To-Use IHC Kit. HBB primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67899-1-Ig targets HBB in WB, ELISA applications and shows reactivity with Human, rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, rabbit |
| 免疫原 | HBB fusion protein Ag9216 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | hemoglobin, beta |
| 别名 | beta globin, CD113t C, HBB, Hemoglobin beta chain, Hemoglobin subunit beta, hemoglobin, beta |
| 计算分子量 | 147 aa, 16 kDa |
| 观测分子量 | 14-16 Da |
| GenBank蛋白编号 | BC007075 |
| 基因名称 | HBB |
| Gene ID (NCBI) | 3043 |
| RRID | AB_2918655 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P68871 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HBB antibody 67899-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |