验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse heart tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IF-P detected in | human appendicitis tissue, human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14537-1-AP targets Hemoglobin Alpha in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag6040 Product name: Recombinant human HBA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC050661 Sequence: MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hemoglobin, alpha 1 |
| 别名 | HBA1, Alpha globin, Alpha-globin, HBA2, Hemoglobin a |
| 计算分子量 | 15 kDa |
| 观测分子量 | 14-16 kDa, 27 kDa |
| GenBank蛋白编号 | BC050661 |
| 基因名称 | HBA1 |
| Gene ID (NCBI) | 3039 |
| RRID | AB_2114462 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P69905 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Hemoglobin Alpha antibody 14537-1-AP | Download protocol |
| IP protocol for Hemoglobin Alpha antibody 14537-1-AP | Download protocol |
| WB protocol for Hemoglobin Alpha antibody 14537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Circulation Sickle Trait and Alpha Thalassemia Increase NOS-Dependent Vasodilation of Human Arteries Through Disruption of Endothelial Hemoglobin-eNOS Interactions | ||
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
J Exp Med WDR82-binding long noncoding RNA lncEry controls mouse erythroid differentiation and maturation | ||
Haematologica Therapeutic effects of induced pluripotent stem cells in chimeric mice with beta-thalassemia. | ||
Haematologica Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. | ||
Mol Cells Adherent-to-suspension transition modulates circulating tumor cell dynamics and metastatic potential in melanoma. |
