验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, human placenta tissue, mouse lung tissue, mouse skin tissue |
| Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 2 publications below |
产品信息
20104-1-AP targets HAUS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag13717 Product name: Recombinant human HAUS4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-363 aa of BC002554 Sequence: MASGDFCSPGEGMEILQQVCSKQLPPCNLSKEDLLQNPYFSKLLLNLSQHVDESGLSLTLAKEQAQAWKEVRLHKTTWLRSEILHRVIQELLVDYYVKIQDTNVTSEDKKFHETLEQRLLVTELMRLLGPSQEREIPPLLGLEKADLLELMPLSEDFVWMRARLQQEVEEQLKKKCFTLLCYYDPNSDADSETVKAAKVWKLAEVLVGEQQQCQDAKSQQKEQMLLLEKKSAAYSQVLLRCLTLLQRLLQEHRLKTQSELDRINAQYLEVKCGAMILKLRMEELKILSDTYTVEKVEVHRLIRDRLEGAIHLQEQDMENSRQVLNSYEVLGEEFDRLVKEYTVLKQATENKRWALQEFSKVYR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 14 open reading frame 94 |
| 别名 | C14orf94, HAUS augmin-like complex subunit 4 |
| 计算分子量 | 363 aa, 42 kDa |
| 观测分子量 | 28 kDa, 37 kDa, 42 kDa |
| GenBank蛋白编号 | BC002554 |
| 基因名称 | C14orf94 |
| Gene ID (NCBI) | 54930 |
| RRID | AB_10639366 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H6D7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HAUS4, also named as C14orf94, has four isoforms with MW 27-29 kDa, 37 kDa and 42 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HAUS4 antibody 20104-1-AP | Download protocol |
| WB protocol for HAUS4 antibody 20104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Structural mechanisms for centrosomal recruitment and organization of the microtubule nucleator γ-TuRC | ||
J Cell Biol Reconstitution and mechanistic dissection of the human microtubule branching machinery. | ||
Nat Commun An interaction network of inner centriole proteins organised by POC1A-POC1B heterodimer crosslinks ensures centriolar integrity |
