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HAUS4 Polyclonal antibody

HAUS4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20104-1-AP

Print datasheet

Synonyms

C14orf94, HAUS4

验证数据展示

  • IHC staining of human prostate cancer using 20104-1-AP

    IHC staining of human prostate cancer using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 20104-1-AP

    IHC staining of human prostate cancer using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human brain using 20104-1-AP

    WB analysis of human brain using 20104-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20104-1-AP

    IHC staining of human placenta using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20104-1-AP (HAUS4 antibody) at dilution of 1:100 (under 10x lens).

经过测试的应用

Positive WB detected inhuman brain tissue, human placenta tissue, mouse lung tissue, mouse skin tissue
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20104-1-AP is HAUS4 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HAUS4 fusion protein Ag13717 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 94
Synonyms C14orf94, HAUS4
Calculated Molecular Weight 363 aa, 42 kDa
Observed Molecular Weight 28 kDa, 37 kDa, 42 kDa
GenBank Accession NumberBC002554
Gene Symbol C14orf94
Gene ID (NCBI) 54930
RRIDAB_10639366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6D7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAUS4, also named as C14orf94, has four isoforms with MW 27-29 kDa, 37 kDa and 42 kDa.

实验方案

Product Specific Protocols
WB protocol for HAUS4 antibody 20104-1-APDownload protocol
IHC protocol for HAUS4 antibody 20104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article