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验证数据展示

  • WB analysis using RMX00092

    WB analysis using RMX00092

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00092 (HAT1 antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using RMX00092

    IHC staining of mouse colon using RMX00092

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using RMX00092 (HAT1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HAT1 antibody included
  • IF Staining of HeLa using RMX00092

    IF Staining of HeLa using RMX00092

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HAT1 antibody (RMX00092) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Multi-rAb® HAT1 Multi-Recombinant antibody

HAT1 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00092

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:2.3.1.48, KAT1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, NIH/3T3 cells, PC-12 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HAT1 Ready-To-Use IHC Kit. HAT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00092 targets HAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 histone acetyltransferase 1
别名 EC:2.3.1.48, KAT1
计算分子量 419 aa, 50 kDa
观测分子量 46 kDa
GenBank蛋白编号BC018682
基因名称 HAT1
Gene ID (NCBI) 8520
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDO14929
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAT1 (also known as KAT1) is located on human chromosome 2q31.1 and encodes histone acetyltransferase 1. This gene is widely expressed in various tissues, and the protein is predominantly localized nuclear in normal cells. Its primary function is to acetylate newly synthesized histones H4 (Lys5, Lys12) and H2A (Lys5), thereby promoting chromatin assembly and playing a key role in DNA replication, cell cycle progression, and DNA damage repair. This antibody can detect a band around 46 kDa.

实验方案

Product Specific Protocols
IF protocol for HAT1 antibody RMX00092Download protocol
IHC protocol for HAT1 antibody RMX00092Download protocol
WB protocol for HAT1 antibody RMX00092Download protocol
Standard Protocols
Click here to view our Standard Protocols
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