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HARS2 Polyclonal antibody

HARS2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 11301-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

EC:6.1.1.21, HARSL, HARSR, Histidine--tRNA ligase, mitochondrial, Histidine--tRNA ligase-like

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inMCF7 cells, HEK-293 cells, human heart tissue, MCF-7 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11301-1-AP targets HARS2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag1827

Product name: Recombinant human HARS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 157-506 aa of BC014982

Sequence: WRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histidyl-tRNA synthetase 2, mitochondrial (putative)
别名 EC:6.1.1.21, HARSL, HARSR, Histidine--tRNA ligase, mitochondrial, Histidine--tRNA ligase-like
计算分子量 57 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC014982
基因名称 HARS2
Gene ID (NCBI) 23438
RRIDAB_10640530
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49590
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HARS2, also named as HARSL, HARSR, HO3 and HisRS, belongs to the class-II aminoacyl-tRNA synthetase family. It is an enzyme that catalyzes the esterification of histidine to its cognate tRNA as an early step in protein biosynthesis. When overexpressed in HIV-1-producing cells, HARS2 was incorporated into wild-type virions but not in ones containing the dilysine-mutated variant of matrix(MA) protein . HO3, through its recognition of MA, plays a role in the life cycle of HIV-1. (PMID: 9445076 ,7755634 ). 1-33aa of HARS2 is transit peptide and this part will be cut in some form(~52kd) of HARS2. This antibody can bind both HARSs for the close sequences.

实验方案

Product Specific Protocols
WB protocol for HARS2 antibody 11301-1-APDownload protocol
IP protocol for HARS2 antibody 11301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Cell

Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.

Authors - Yilin Kang
humanWB

J Biol Chem

Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.

Authors - Shasha Gong
mouseWB

Front Cell Neurosci

Disruption of Hars2 in Cochlear Hair Cells Causes Progressive Mitochondrial Dysfunction and Hearing Loss in Mice.

Authors - Pengcheng Xu
  • KO Validated
mouseWB

Cells

CLPP Depletion Causes Diplotene Arrest; Underlying Testis Mitochondrial Dysfunction Occurs with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2

Authors - Jana Key
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