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验证数据展示

  • WB analysis using 67662-1-Ig (same clone as 67662-1-PBS)

    WB analysis using 67662-1-Ig (same clone as 67662-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67662-1-Ig (HARS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67662-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67662-1-Ig (same clone as 67662-1-PBS)

    IF Staining of MCF-7 using 67662-1-Ig (same clone as 67662-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HARS antibody (67662-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67662-1-PBS in a different storage buffer formulation.

HARS Monoclonal antibody, PBS Only

HARS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67662-1-PBS

产品说明书

CloneNo. 1B7G2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

FLJ20491, HARS, HisRS, Histidine tRNA ligase, histidyl tRNA synthetase, HRS

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67662-1-PBS targets HARS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag9224

Product name: Recombinant human HARS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-282 aa of BC011807

Sequence: MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 histidyl-tRNA synthetase
别名 FLJ20491, HARS, HisRS, Histidine tRNA ligase, histidyl tRNA synthetase, HRS
计算分子量 509 aa, 57 kDa
观测分子量 50 kDa
GenBank蛋白编号BC011807
基因名称 HARS
Gene ID (NCBI) 3035
RRIDAB_2882859
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP12081
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

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