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验证数据展示

  • WB analysis using 84482-4-RR

    WB analysis using 84482-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84482-4-RR (HARBI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84482-4-RR

    Affinity and Kinetic Characterization of 84482-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84482-4-RR against Human HARBI1 were performed. The affinity constant is 78.4 pM.

HARBI1 Recombinant antibody

HARBI1 Recombinant Antibody for WB, ELISA
Cat No. 84482-4-RR

产品说明书

CloneNo. 241915C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Putative nuclease HARBI1, Harbinger transposase-derived nuclease, EC:3.1.-.-, C11orf77, 241915C10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, Jurkat cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84482-4-RR targets HARBI1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 HARBI1 fusion protein Ag34137 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 harbinger transposase derived 1
别名 Putative nuclease HARBI1, Harbinger transposase-derived nuclease, EC:3.1.-.-, C11orf77, 241915C10
计算分子量 349 aa, 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC036925
基因名称 HARBI1
Gene ID (NCBI) 283254
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96MB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HARBI1, also named as harbinger transposase derived 1, is predicted to enable metal ion binding activity and nuclease activity. HARBI1 is predicted to be involved in nucleic acid phosphodiester bond hydrolysis. [provided by Alliance of Genome Resources, Apr 2022] In plants, HARBI1 genes have developed new cellular functions to benefit the host, certain HARBI1 genes in rice and Arabidopsis thaliana ( A. thaliana) have been reported to play critical roles in regulating plant growth and development.(PMID:37950159)

实验方案

Product Specific Protocols
WB protocol for HARBI1 antibody 84482-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols