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验证数据展示

  • WB analysis of mouse brain using 21228-1-AP

    WB analysis of mouse brain using 21228-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21228-1-AP (HAPLN4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 21228-1-AP

    IHC staining of human brain using 21228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21228-1-AP (HAPLN4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 21228-1-AP

    IHC staining of human brain using 21228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21228-1-AP (HAPLN4 antibody) at dilution of 1:100 (under 40x lens).

HAPLN4 Polyclonal antibody

HAPLN4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21228-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, ELISA

别名

Brain link protein 2, BRAL2, HAPLN4, KIAA1926

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

21228-1-AP targets HAPLN4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse, zebrafish
免疫原

CatNo: Ag15687

Product name: Recombinant human HAPLN4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-206 aa of BC142644

Sequence: QRGRKKVVHVLEGESGSVVVQTAPGQVVSHRGGTIVLPCRYHYEAAAHGHDGVRLKWTKVVDPLAFTDVFVALGPQHRAFGSYRGRAELQGDGPGDASLVLRNVTLQDYGRYECEVTNELEDDAGMVKLDLEGVVFPYHPRGGRYKLTFAEAQRACAEQDGILASAEQLHAAWRDGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hyaluronan and proteoglycan link protein 4
别名 Brain link protein 2, BRAL2, HAPLN4, KIAA1926
计算分子量 402 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC142644
基因名称 HAPLN4
Gene ID (NCBI) 404037
RRIDAB_10695504
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UW8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HAPLN4 antibody 21228-1-APDownload protocol
IHC protocol for HAPLN4 antibody 21228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Diabetes

Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.

Authors - Yuguo Niu
View Article
zebrafishWB

bioRxiv

MMP-2/9 inhibition modulates sharp wave abundance, inhibitory proteoglycan sulfation, and fear memory in juvenile zebrafish: relevance to affective disorders

Authors - I Blanco
View Article