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验证数据展示

  • WB analysis of mouse liver using 25056-1-AP

    WB analysis of mouse liver using 25056-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25056-1-AP (HAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

HAO1 Polyclonal antibody

HAO1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25056-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Glycolate oxidase, GOX, GOX1, HAO1, HAOX1, Hydroxyacid oxidase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25056-1-AP targets HAO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21884

Product name: Recombinant human HAO1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-260 aa of BC113665

Sequence: MLPRLICINDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGDDSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHGLNGILVSNH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxyacid oxidase (glycolate oxidase) 1
别名 Glycolate oxidase, GOX, GOX1, HAO1, HAOX1, Hydroxyacid oxidase 1
计算分子量 370 aa, 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC113665
基因名称 HAO1
Gene ID (NCBI) 54363
RRIDAB_2879875
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJM8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HAO1 antibody 25056-1-APDownload protocol
IHC protocol for HAO1 antibody 25056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols