验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10882-1-AP targets HAND1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag1324 Product name: Recombinant human HAND1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC021190 Sequence: MNLVGSYAHHHHHHHPHPAHPMLHEPFLFGPASRCHQERPYFQSWLLSPADAAPDFPAGGPPPAAAAAATAYGPDARPGQSPGRLEALGGRLGRRKGSGPKKERRRTESINSAFAELRECIPNVPADTKLSKIKTLRLATSYIAYLMDVLAKDAQSGDPEAFKAELKKADGGRESKRKRELQQHEGFPPALGPVEKRIKGRTGWPQQVWALELNQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heart and neural crest derivatives expressed 1 |
| 别名 | bHLHa27, Class A basic helix-loop-helix protein 27, eHAND, Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1, Heart- and neural crest derivatives-expressed protein 1 |
| 计算分子量 | 24 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC021190 |
| 基因名称 | HAND1 |
| Gene ID (NCBI) | 9421 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O96004 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The HAND1 gene encodes a basic helix-loop-helix (bHLH) transcription factor essential for mammalian heart development (PMID:19586923). HAND1 is highly expressed in the heart and in cytotrophoblastic cell lines, and is required in the adult for sustained expression of cardiac-specific genes (PMID:9931445).
