验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue, mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86771-3-RR targets HAL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag22985 Product name: Recombinant human HAL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC096097 Sequence: MPRYTVHVRGEWLAVPCQDAQLTVGWLGREAVRRYIKNKPDNGGFTSVDDAHFLVRRCKGLGLLDNEDRLEVALENNEFVEVVIEGDAMSPDFIPSQPEGVYLYSKYREPEKYIELDGDRLTTEDLVNLGKGRYKIKLTPTAEKRVQKSREVIDSIIKEKTVVYGITTGFGKFARTVIPINKLQELQVNLVRSHSSGVGKPLSPERCRMLLALRINVLAKGYSGISLETLKQVIEMFNASCLPYVPEKGTVGASGDLAPLSHLALGLVGEGKMWSPKSGWADAKYVLEAHGLKPVILKPKEGLALINGTQMITSLGCEAVERASAIARQADIVAALTLEVLKGTTKAFDTDIHAL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histidine ammonia-lyase |
| 别名 | EC:4.3.1.3, HIS, Histidase, histidine ammonia lyase, Histidine ammonia-lyase |
| 计算分子量 | 657 aa, 73 kDa |
| 观测分子量 | 73 kDa |
| GenBank蛋白编号 | BC096097 |
| 基因名称 | HAL |
| Gene ID (NCBI) | 3034 |
| RRID | AB_3745110 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P42357 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HAL, also named as Histidase and HIS, catalyzes the nonoxidative elimination of the α-amino group of histidine. It is closely related to the important plant enzyme phenylalanine ammonia-lyase. HAL has some isoforms with MW 73 kDa, 65 kDa and 49 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HAL antibody 86771-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
