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HADHB Monoclonal antibody

HADHB Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D12F4

Cat No : 67967-1-Ig

Print datasheet

Synonyms

Acetyl CoA acyltransferase, Beta ketothiolase, ECHB, HADHB, MSTP029, TP BETA

验证数据展示

  • WB analysis using 67967-1-Ig

    WB analysis using 67967-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67967-1-Ig (HADHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 67967-1-Ig

    WB analysis using 67967-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67967-1-Ig (HADHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67967-1-Ig

    IHC staining of human colon cancer using 67967-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67967-1-Ig (HADHB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HADHB antibody included
  • IF Staining of HeLa using 67967-1-Ig

    IF Staining of HeLa using 67967-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHB antibody (67967-1-Ig, Clone: 1D12F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 67967-1-Ig

    IF Staining of HeLa using 67967-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHB antibody (67967-1-Ig, Clone: 1D12F4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inLNCaP cells, pig heart tissue, Hela cells, HEK-293 cells, HepG2 cells, Jurkat cells, Rabbit heart tissues, Rat heart tissues, Mouse heart tissues
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HADHB Ready-To-Use IHC Kit. HADHB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67967-1-Ig targets HADHB in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen HADHB fusion protein Ag30315 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit
Synonyms Acetyl CoA acyltransferase, Beta ketothiolase, ECHB, HADHB, MSTP029, TP BETA
Calculated Molecular Weight 51 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC017564
Gene Symbol HADHB
Gene ID (NCBI) 3032
RRIDAB_2918718
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55084
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HADHB, also named as TP- beta, Acetyl-CoA acyltransferase and Beta-ketothiolase, is a mitochondrial trifunctional enzyme subunit beta. Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity. HADHB has 2 isoforms produced by alternative splicing with the MW of 49 kDa and 51 kDa.

实验方案

Product Specific Protocols
WB protocol for HADHB antibody 67967-1-IgDownload protocol
IHC protocol for HADHB antibody 67967-1-IgDownload protocol
IF protocol for HADHB antibody 67967-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols