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  • Featured Product
  • KD/KO Validated

HACL1 Polyclonal antibody

HACL1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15519-1-AP

Print datasheet

Synonyms

2 HPCL, 2 hydroxyacyl CoA lyase 1, 2 hydroxyphytanoyl CoA lyase, HACL1, HPCL, HPCL2, PHYH2, Phytanoyl CoA 2 hydroxylase 2

验证数据展示

  • IP experiment of HeLa using 15519-1-AP

    IP experiment of HeLa using 15519-1-AP

    IP result of anti-HACL1 (IP:15519-1-AP, 4ug; Detection:15519-1-AP 1:300) with HeLa cells lysate 2640 ug.

  • WB analysis of HeLa using 15519-1-AP

    WB analysis of HeLa using 15519-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15519-1-AP (HACL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 15519-1-AP

    WB analysis of HEK-293 using 15519-1-AP

    WB result of HACL1 antibody (15519-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HACL1 transfected HEK-293 cells.

  • IHC staining of rat liver using 15519-1-AP

    IHC staining of rat liver using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15519-1-AP (HACL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 15519-1-AP

    IHC staining of rat liver using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15519-1-AP (HACL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15519-1-AP

    IF Staining of HeLa using 15519-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15519-1-AP (HACL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inrat liver tissue, human liver tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15519-1-AP targets HACL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen HACL1 fusion protein Ag7830 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 2-hydroxyacyl-CoA lyase 1
Synonyms 2 HPCL, 2 hydroxyacyl CoA lyase 1, 2 hydroxyphytanoyl CoA lyase, HACL1, HPCL, HPCL2, PHYH2, Phytanoyl CoA 2 hydroxylase 2
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC001627
Gene Symbol HACL1
Gene ID (NCBI) 26061
RRIDAB_2279502
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJ83
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HACL1 antibody 15519-1-APDownload protocol
IHC protocol for HACL1 antibody 15519-1-APDownload protocol
IF protocol for HACL1 antibody 15519-1-APDownload protocol
IP protocol for HACL1 antibody 15519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols