验证数据展示
经过测试的应用
| Positive WB detected in | A-204 cells, DU 145 cells, HepG2 cells, PANC-1 cells, SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33317-1-AP targets HABP4 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag39922 Product name: Recombinant human HABP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 280-413 aa of NM_014282.3 Sequence: LEVEEETQVQEMTLDEWKNLQEQTRPKPEFNIRKPESTVPSKAVVIHKSKYRDDMVKDDYEDDSHVFRKPANDITSQLEINFGNLPRPGRGARGGTRGGRGRIRRAENYGPRAEVVMQDVAPNPDDPEDFPALS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hyaluronan binding protein 4 |
| 别名 | Hyaluronan-binding protein 4, IHABP4, IHABP-4, Intracellular hyaluronan-binding protein 4, Ki-1/57 intracellular antigen |
| 计算分子量 | 46kDa，413aa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | NM_014282.3 |
| 基因名称 | HABP4 |
| Gene ID (NCBI) | 22927 |
| RRID | AB_3742938 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q5JVS0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HABP4 (hyaluronic acid binding protein 4) is an intracellular regulatory protein, with a molecular weight of about 57 kDa (PMID: 33245729), which is rich in positively charged amino acids and can bind with negatively charged macromolecules such as RNA and hyaluronic acid. It has SUMO binding activity, participates in PML tissue, negatively regulates DNA binding activity, and positively regulates gene expression. In addition, HABP4 has a complex interaction network in cells, and has undergone a variety of post-translational modifications, including serine/threonine phosphorylation, arginine methylation and SUMO.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HABP4 antibody 33317-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
