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验证数据展示

  • WB analysis using 86673-1-RR (same clone as 86673-1-PBS)

    WB analysis using 86673-1-RR (same clone as 86673-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86673-1-RR (HAAO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86673-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86673-1-RR (same clone as 86673-1-PBS)

    IF Staining of HepG2 using 86673-1-RR (same clone as 86673-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HAAO antibody (86673-1-RR, Clone: 251701F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86673-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86673-1-RR

    Affinity and Kinetic Characterization of 86673-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86673-1-RR against Human HAAO were performed. The affinity constant is 8.17 nM.

HAAO Recombinant monoclonal antibody, PBS Only

HAAO Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86673-1-PBS

产品说明书

CloneNo. 251701F11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

3 HAO, 3-HAO, 3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilic acid dioxygenase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86673-1-PBS targets HAAO in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3511

Product name: Recombinant human HAAO protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-286 aa of BC029510

Sequence: MERRLGVRAWVKENRGSFQPPVCNKLMHQEQLKVMFIGGPNTRKDYHIEEGEEVFYQLEGDMVLRVLEQGKHRDVVIRQGEIFLLPARVPHSPQRFANTVGLVVERRRLETELDGLRYYVGDTMDVLFEKWFYCKDLGTQLAPIIQEFFSSEQYRTGKPIPDQLLKEPPFPLSTRSIMEPMSLDAWLDSHHRELQAGTPLSLFGDTYETQVIAYGQGSSEGLRQNVDVWLWQLEGSSVVTMGGRRLSLAPDDSLLVLAGTSYAWERTQGSVALSVTQDPACKKPLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 3-hydroxyanthranilate 3,4-dioxygenase
别名 3 HAO, 3-HAO, 3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilic acid dioxygenase
计算分子量 286 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC029510
基因名称 HAAO
Gene ID (NCBI) 23498
RRIDAB_3745045
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP46952
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HAAO is also known by several synonyms, including 3-HAO (3-hydroxyanthranilate oxygenase), HAO (3-hydroxyanthranilic acid dioxygenase), h3HAO and HAD. HAAO is widely distributed in peripheral organs, with particularly high expression in the liver and kidney. Strong positivity has been observed in the cytoplasm of hepatocytes and renal tubules, as well as in cilia of respiratory epithelium and fallopian tube epithelium. Moderate staining is detected in the small intestine and subsets of lymphoid cells.

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