验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, rat liver tissue, mouse liver tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
产品信息
12791-1-AP targets HAAO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3511 Product name: Recombinant human HAAO protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-286 aa of BC029510 Sequence: MERRLGVRAWVKENRGSFQPPVCNKLMHQEQLKVMFIGGPNTRKDYHIEEGEEVFYQLEGDMVLRVLEQGKHRDVVIRQGEIFLLPARVPHSPQRFANTVGLVVERRRLETELDGLRYYVGDTMDVLFEKWFYCKDLGTQLAPIIQEFFSSEQYRTGKPIPDQLLKEPPFPLSTRSIMEPMSLDAWLDSHHRELQAGTPLSLFGDTYETQVIAYGQGSSEGLRQNVDVWLWQLEGSSVVTMGGRRLSLAPDDSLLVLAGTSYAWERTQGSVALSVTQDPACKKPLG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 3-hydroxyanthranilate 3,4-dioxygenase |
| 别名 | 3 HAO, 3-HAO, 3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilic acid dioxygenase |
| 计算分子量 | 286 aa, 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC029510 |
| 基因名称 | HAAO |
| Gene ID (NCBI) | 23498 |
| RRID | AB_2115432 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P46952 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HAAO is also known by several synonyms, including 3-HAO (3-hydroxyanthranilate oxygenase), HAO (3-hydroxyanthranilic acid dioxygenase), h3HAO and HAD. HAAO is widely distributed in peripheral organs, with particularly high expression in the liver and kidney. Strong positivity has been observed in the cytoplasm of hepatocytes and renal tubules, as well as in cilia of respiratory epithelium and fallopian tube epithelium. Moderate staining is detected in the small intestine and subsets of lymphoid cells.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HAAO antibody 12791-1-AP | Download protocol |
| IHC protocol for HAAO antibody 12791-1-AP | Download protocol |
| WB protocol for HAAO antibody 12791-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth
| ||
BMC Genomics Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats | ||
Nat Commun Niacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma | ||
Environ Pollut Bisphenol F exposure induces depression-like changes: Roles of the kynurenine metabolic pathway along the "liver-brain" axis | ||
Gut Microbes Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via Akkermansia muciniphila | ||
Acta Pharm Sin B The crucial function of IDO1 in pulmonary fibrosis: From the perspective of mitochondrial fusion in lung fibroblasts and targeted molecular inhibition. |
