验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, rat liver tissue, mouse liver tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
产品信息
12791-1-AP targets HAAO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3511 Product name: Recombinant human HAAO protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-286 aa of BC029510 Sequence: MERRLGVRAWVKENRGSFQPPVCNKLMHQEQLKVMFIGGPNTRKDYHIEEGEEVFYQLEGDMVLRVLEQGKHRDVVIRQGEIFLLPARVPHSPQRFANTVGLVVERRRLETELDGLRYYVGDTMDVLFEKWFYCKDLGTQLAPIIQEFFSSEQYRTGKPIPDQLLKEPPFPLSTRSIMEPMSLDAWLDSHHRELQAGTPLSLFGDTYETQVIAYGQGSSEGLRQNVDVWLWQLEGSSVVTMGGRRLSLAPDDSLLVLAGTSYAWERTQGSVALSVTQDPACKKPLG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 3-hydroxyanthranilate 3,4-dioxygenase |
| 别名 | EC:1.13.11.6, 3-hydroxyanthranilic acid dioxygenase, 3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilate 3,4-dioxygenase, 3-HAO |
| 计算分子量 | 286 aa, 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC029510 |
| 基因名称 | HAAO |
| Gene ID (NCBI) | 23498 |
| RRID | AB_2115432 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P46952 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HAAO antibody 12791-1-AP | Download protocol |
| IHC protocol for HAAO antibody 12791-1-AP | Download protocol |
| WB protocol for HAAO antibody 12791-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth
| ||
BMC Genomics Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats | ||
Nat Commun Niacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma | ||
Environ Pollut Bisphenol F exposure induces depression-like changes: Roles of the kynurenine metabolic pathway along the "liver-brain" axis | ||
Gut Microbes Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via Akkermansia muciniphila |
