验证数据展示
经过测试的应用
| Positive WB detected in | Transfected HEK-293 cells, HEK-293 cells, Ag19172 |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66006-2-Ig targets HA Tag in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, PLA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP, ChIP, RIP, ELISA, PLA |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | human, mouse, rat, recombinant protein |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | HA Tag |
| 别名 | YPYDVPDYA |
| 计算分子量 | 1 kDa |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2881490 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | HATAG |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| IF protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| IP protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| WB protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Aberrant Notch-signaling promotes tumor angiogenesis in esophageal squamous-cell carcinoma | ||
J Hematol Oncol METTL16-SENP3-LTF axis confers ferroptosis resistance and facilitates tumorigenesis in hepatocellular carcinoma | ||
Science DNA-protein cross-links promote cGAS-STING-driven premature aging and embryonic lethality. |
