Phospho-Histone H3 (Ser10) Monoclonal antibody
Phospho-Histone H3 (Ser10) Monoclonal Antibody for FC, IF/ICC,IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse, pig and More (2)
Applications
FC, IF/ICC,IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
4C7G2
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells |
Positive IHC detected in | human lung cancer tissue, Jurkat cells, human renal cell carcinoma tissue, mouse kidney tissue, rat kidney tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human breast cancer tissue, HeLa cells, A549 cells, MCF-7 cells, SKOV-3 cells, mouse testis tissue, C2C12 cells |
Positive FC detected in | HepG2 cells, nocodazole treated HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66863-1-Ig targets Phospho-Histone H3 (Ser10) in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, rat, mouse, pig samples.
Tested Applications | FC, IF/ICC,IF-P, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | Human, rat, mouse, pig |
Cited Reactivity | human, mouse, rat, chicken |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | histone cluster 1, H3a |
Synonyms | H3 S10, H3 Ser10, H3/A, H3FA, H3S10, HIST1H3A, histone, histone cluster 1, H3a, Histone H3.1, Histone H3/a, PHH3, Phospho-Histone H3 Ser10 |
Calculated Molecular Weight | 15 kDa |
Observed Molecular Weight | 15-17 kDa |
GenBank Accession Number | NM_003529 |
Gene Symbol | HIST1H3A |
Gene ID (NCBI) | 8350 |
RRID | AB_2882201 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P68431 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Phospho-Histone H3 (Ser10) antibody 66863-1-Ig | Download protocol |
IHC protocol for Phospho-Histone H3 (Ser10) antibody 66863-1-Ig | Download protocol |
IF protocol for Phospho-Histone H3 (Ser10) antibody 66863-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oncogene Oncogenic long intervening noncoding RNA Linc00284 promotes c-Met expression by sponging miR-27a in colorectal cancer. | ||
Biochem Pharmacol A novel aromatic amide derivative SY-65 co-targeted tubulin and histone deacetylase 1 with potent anticancer activity in vitro and in vivo. | ||
Front Mol Biosci High-Resolution Imaging of Tumor Spheroids and Organoids Enabled by Expansion Microscopy. | ||
Sci Rep NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway. | ||
Dev Dyn The dynamic spatial and temporal relationships between the phalanx-forming region and the interdigits determine digit identity in the chick limb autopod. | ||
Front Immunol Oocytes could rearrange immunoglobulin production to survive over adverse environmental stimuli |