验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, mouse kidney tissue |
| Positive IHC detected in | human skin cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16441-1-AP targets Histone H2A.z in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, zebrafish, fish, oratosquilla oratoria |
| 免疫原 |
CatNo: Ag9762 Product name: Recombinant human Histone H2A.z protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC018002 Sequence: MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | H2A histone family, member Z |
| 别名 | H2A, H2AFZ, H2A.z, H2A/z, H2AZ |
| 计算分子量 | 128 aa, 14 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC018002 |
| 基因名称 | Histone H2A.z |
| Gene ID (NCBI) | 3015 |
| RRID | AB_2115113 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P0C0S5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Histone H2A.z antibody 16441-1-AP | Download protocol |
| IHC protocol for Histone H2A.z antibody 16441-1-AP | Download protocol |
| WB protocol for Histone H2A.z antibody 16441-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun NPM3 functions as a lactyltransferase to promote necroptosis in male diabetic cardiomyopathy mice models via FASN transcription modulation. | ||
Exp Mol Med Keratin 18 functions as a lactyltransferase to trigger necroptosis in diabetic kidney disease by modulating Fas transcription. | ||
Nat Chem Biol Designing chemigenetic DNA nanotrap for norepinephrine dynamic imaging in organelles. | ||
Adv Sci (Weinh) Nap1L4a Cooperates with Scl/Klf1 to Recruit H2A.Z in Mediating Interactions Among Cis-Regulatory Elements and Transcription Required for Primitive Erythropoiesis in Zebrafish. | ||
Adv Sci (Weinh) Histone Lactylation-Driven Upregulation of VRK1 Expression Promotes Stemness and Proliferation of Glioma Stem Cells | ||
